MARS DIAGNOSTIC PLATFORM
SPI Analyzer
SPI Analyzer uses 3-dimensional magnetic particle tomography to image the analytical regions of assay chips and quantify the SPMP labeled analytes immobilized in the regions. It also uses the harmonics generated by Brownian and Neel relaxation of the SPMP to differentiate analytes labeled with different SPMPs. The SPI Analyzer is equipped with GPS, Wi-Fi, Bluetooth, Ethernet and USB ports. The GPS and network capability enable reporting the complete results with time and location stamps in real time. It can be easily integrated into hospital and clinic databases or any other system. Because of the superior SPI technology, this instrument has sensitivity at the single digit picogram level. The instrument can be connected to Mars Mobile App through Bluetooth and download the results to mobile devices.
Mars Mobile App
The Mars Mobile App is fully integrated with the Mars Analyzer. It can automatically download test results from an analyzer and generates reports for doctors and patients. It displays test results and informs you if they are normal, high, or low. Those results are color coded. It also suggests potential treatments. It can store and plot historical data as well as overlay historical charts from different tests for a comprehensive diagnosis. The Mars Mobile App can also send reports using applications available on the mobile device. It is also capable of managing in-app purchases.
HY-POC Chip
HY-POC is a patented technology that takes advantage of many different formats such as lateral flow tests, microfluidics, Elisa assays and others without the specific issues associated with those formats.Because of its 3D design, it can simultaneously run assays that are not compatible among each other. Due to the high sensitivity of SPI technology, only 10 µL of sample is needed for analysis, which makes finger prick sampling possible. With the HY-POC technology, the run time for each assay is less than 10 minutes. Most assays are complete in less than 5 minutes.
Highlights
- Rapid assay (5 minutes)
- Small sample size (10 µL), capable of finger prick sampling
- High sensitivity (picogram level), low interference
- Quantitative results, low CV (<10%)
- Portable size (8 x 6 x 4 in, 2 lb.)
- Multidimensional multiplexing capability for measuring a panel of analytes on a single chip
- Easy to operate, little to no sample preparation for most analyses
- Stable and easy to handle (long shelf life, room temperature use and storage)
- Lithium battery powered (up to 8 hours of operation time)
- Built-in data communication and network capability (Wi-Fi, Bluetooth, Ethernet, USB, 2D barcode reader)
- Built-in GPS
- Fully integrated Mars Mobile App for smart phones and tablets
ABOUT MARS Sciences
The Company
Mars Sciences LLC is a startup company engaged in research and development of the next generation of diagnostic devices based on patented Superparamagnetic Particle Imaging technologies and their applications in in-vitro diagnostic, environmental analysis, chemical and biological warfare, blood banking, drug of abuse detection, pandemic monitoring, food safety and much more
Our Technology
Superparamagnetic Particle Imaging (SPI) is 3-dimentional superparamagnetic particle tomography. It uses non-linear response of superparamagnetic nanoparticles (SPMP) to the change of external applied magnetic field to achieve multidimensional multiplex quantification of analytes immobilized in an analytical region.
MARS Diagnostic System By The Numbers
Schematic Description of the SPI Analyzer Work Flow
Initial Setup
In this particular example, the assay chip has one analytical region which consists of 3 compartments A, B, and C. Each compartment is immobilized with two capturing antibodies specific to Analyte 1&2, 3&4, and 5&6 respectively. The sample port contains 2 different types of SPMPs (SPMP 1, black round beads and SPMP 2, blue oval beads) conjugated with 6 recognition antibodies specific to analytes 1, 3, 5 and 2, 4, 6 respectively. 10 l of sample containing 6 different analytes are ready to be added (Fig. 1).
Fig. 1 The configuration of SPI Analyzer and assay chip prior to sample addition
Running Assay
10 µl of sample is added to the sample port. It mixes with antibody conjugated SPMPs and travels through the channels automatically. After assay is complete (typically 3-5 minutes), Analytes 1&2, 3&4, and 5&6 are captured in compartments A, B, and C respectively. The two analytes in each compartment are labeled with SPMP 1 and SPMP 2 respectively. The excess of SPMPs are absorbed in the Waste Chamber (Fig. 2).
Fig. 2 The configuration of SPI Analyzer and assay chip after assay is complete
Quantification
SPI Analyzer scans the entire analytical region and reconstructs the total concentration images of each compartment. The computer then calculates the concentrations of SPMP 1 and SPMP 2 using the equations described in 2.2.1 based on the harmonic signals. The results are used to reconstruct the images of each compartment based on SPMP 1 or SPMP 2 exclusively (Fig. 3 and 4). In each of the images, only the analytes labeled with the same SPMP are displayed in terms of their concentrations. In this process, all 6 analytes with unknown concentrations in 10 µl sample were separated and quantified.
Fig. 3 SPI analyzer images the SPMP 1 labeled analytes in the analytical region
Fig. 4 SPI analyzer images the SPMP 2 labeled analytes in the analytical region
Multidimensional Multiplexing
Assuming there are X analytical regions on each assay chip, Y compartments in each region, and Z types of SPMPs used for labeling, one can achieve three dimensions of multiplexing independently. A total of X x Y x Z analytes can be analyzed with a single chip.
Facilities
Assay Development Lab
Chemistry Lab
Chip Developement Lab
CONTACT INFO
EMAIL US
info@marssciences.com
CALL US
+1 (858) 610-0903
